Isolation of Ovine Multipotent Mesenchymal Stem Cells from Umbilical Cord Tissue Wharton’s Jelly
نویسندگان
چکیده
In this study, we tried explant method and a combination of explant-enzymatic method for the extraction of Mesenchymal Stem Cells from ovine umbilical cord tissue wharton’s jelly. The isolated and culture expanded cells expressed strong alkaline phosphatase activity and they were shown to form colonies. The Mesenchymal Stem Cells showed positive for CD44 and negative for CD34 surface markers by ow cytometry. These ndings indicated that the cells with MSC phenotype can be isolated much faster and higher in mixed explant-enzymatic method from ovine UCT-WJ.
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